- Conference room with glass case of reliquaries with the collection of mid 17th century bed-head paintings in the background
- Some mid 17th century bed-head paintings representing bunches of flowers in vases with the emblem of the Fatebenefratelli Order (Do Good Brothers or Brothers Hospitallers of Saint John of God)
- 18th century reliquary of S. Giovanni di Dio in gilded and silver bronze
- A room of the museum
- A room of the museum
- Medical instruments and artistic testimonials
- On January 9th 1872 Egisto Cipriani, a 22 year old upholsterer, was joking about with some friends and managed to swallow a fork of 22cm in length. He was taken to the Hospital of S. Giovanni di Dio and then to that of Santa Maria Nuova, but all attempts to find a solution were in vain, because the foreign body could not be found. The young man was sent home and lived a normal life until April 1887 when, suffering from severe pain in his lower abdomen, he was successfully operated on at S. Giovanni Dio. This case attracted so much interest in Italy and Europe that Egisto was nicknamed The fork man.
- The corridor with the cases displaying medical instruments
- The exhibition corridor, previously part of the original ward of the hospital of 1730
- The exhibition corridor showing part of the 17th-18th century collection of portraits of the hospital rectors
- Library Reading Room
- Library Reading Room showing open pages of one of the 19th century medical books
- Meeting Room
- Meeting Room with the 18th century candlesticks in the shape of two fauns in gilded wood
- Medical instruments and furnishings|Medical instruments and furnishings displayed along one side of the loggia on the ground floor that opens onto the courtyard